Latex agglutination assays

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Click on image for details. The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira by culture has a low sensitivity and the microscopic agglutination test MAT is time consuming To overcome these problems, a rapid latex agglutination test LAT has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis.

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A latex agglutination test LAT based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the hemagglutination inhibition test, the sensitivity and specificity of the LAT were The test has application potential in field practice.

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Latex agglutination assays A latex fixation test, also called a latex agglutination assay or test LA assay or testis an assay used clinically in the identification and typing of many important microorganisms. These tests use the patient's antigen-antibody immune response. This response occurs when the body detects a pathogen and forms an antibody specific to an identified antigen a protein configuration present on the surface of the pathogen.

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The latex agglutination test is a laboratory method to check for certain antibodies or antigens in a variety of body fluids including saliva, urine, cerebrospinal fluid, or blood. The sample is sent to a lab, where it is mixed with latex beads coated with a specific antibody or antigen. If the suspected substance is present, the latex beads will clump together agglutinate.

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A group of passive agglutination tests carried out by coating either antigen or antibody on an artificial carrier particle, called latex bead are called as latex agglutination test. They may alternatively referred to as latex fixation test and may be:. To detect microbial and viral infections, autoimmune diseases, hormones, drugs or serum proteins by agglutination reaction of antigen-antibody.

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In addition to causing precipitation of soluble molecules and flocculation of molecules in suspension, antibodies can also clump together cells or particles e. Agglutination can be used as an indicator of the presence of antibodies against bacteria or red blood cells. Microtiter plates have an array of wells to hold small volumes of reagents and to observe reactions e.

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Agglutination is the process that occurs when an antigen is mixed with its corresponding antibody called agglutinin and is commonly used in blood grouping. Latex agglutination test, also called latex fixation, is a diagnostic study that is widely used as a laboratory method to identify certain antibodies and antigens. The test uses a variety of body fluids including blood, urine, saliva and cerebrospinal fluid, which are dependable on what type of sample is needed.

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Click on image for details. Correspondence Address : Dr. Kim KS. Acute bacterial meningitis in infants and children.

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A latex fixation testalso called a latex agglutination assay or test LA assay or testis an assay used clinically in the identification and typing of many important microorganisms. These tests use the patient's antigen - antibody immune response. This response occurs when the body detects a pathogen and forms an antibody specific to an identified antigen a protein configuration present on the surface of the pathogen.

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The latex agglutination test is a laboratory method to check for certain antibodies or antigens in a variety of bodily fluids including saliva, urine, cerebrospinal fluid, or blood. The sample is sent to a lab, where it is mixed with latex beads coated with a specific antibody or antigen. If the suspected substance is present, the latex beads will clump together agglutinate.

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